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plasmid pcris pitchv2 puro dtag  (Addgene inc)


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    Structured Review

    Addgene inc plasmid pcris pitchv2 puro dtag
    Plasmid Pcris Pitchv2 Puro Dtag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcris+pitchv2+puro+dtag/pCRIS-PITChv2-Puro-dTAG+(BRD4)+(Plasmid+%2391793)/pm41904131-261-58-60
    Average 93 stars, based on 36 article reviews
    plasmid pcris pitchv2 puro dtag - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells
    Article Snippet: .. pCRIS-PITChv2-Puro-dTAG , Addgene , Plasmid#91793. .. pCRIS-PITChv2-BSD-dTAG , Addgene , Plasmid#91792.

    Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles
    Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS- FB2). .. Generating SON, SRRM2, and CTCF knock-in cell lines To generate an N- terminal Puro- P2A- dtag SON knock- in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro- P2A- dTAG, subcloned from pCRIS- PITChv2- Puro- dTAG, a gift from J Bradner and B Nabet (Addgene # 91793). ..

    Article Title: Targeting the mSWI/SNF complex in POU2F-POU2AF transcription factor-driven malignancies
    Article Snippet: LentiV_Cas9_puro , Tarumoto et al. , Addgene Plasmid #108100. .. pCRIS-PITChv2-Puro-dTAG , Nabet et al. , Addgene Plasmid #91793. .. hPGK-POU2AF1-HAx3-Puro , Vectorbuilder , N/A.

    Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles
    Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS-FB2). .. To generate an N-terminal Puro-P2A-dtag SON knock-in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro-P2A-dTAG, subcloned from pCRIS-PITChv2-Puro-dTAG, a gift from J Bradner and B Nabet (Addgene # 91793). ..

    Generated:

    Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells
    Article Snippet: .. Degron cell lines were generated following.31,32 Briefly, Kdm3a microhomology arms were added to the pCRIS-PITChv2-Puro-dTAG (Addgene #91793) donor vector and Kdm3b microhomology arms were cloned into the pCRIS-PITChv2-BSD-dTAG (Addgene #91792) donor vector to allow for dual selection with puromycin and blasticidin S hydrochloride. ..

    Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells
    Article Snippet: .. Degron cell lines were generated following., Briefly, Kdm3a microhomology arms were added to the pCRIS-PITChv2-Puro-dTAG (Addgene #91793) donor vector and Kdm3b microhomology arms were cloned into the pCRIS-PITChv2-BSD-dTAG (Addgene #91792) donor vector to allow for dual selection with puromycin and blasticidin S hydrochloride. ..

    Clone Assay:

    Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells
    Article Snippet: .. Degron cell lines were generated following.31,32 Briefly, Kdm3a microhomology arms were added to the pCRIS-PITChv2-Puro-dTAG (Addgene #91793) donor vector and Kdm3b microhomology arms were cloned into the pCRIS-PITChv2-BSD-dTAG (Addgene #91792) donor vector to allow for dual selection with puromycin and blasticidin S hydrochloride. ..

    Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells
    Article Snippet: .. Degron cell lines were generated following., Briefly, Kdm3a microhomology arms were added to the pCRIS-PITChv2-Puro-dTAG (Addgene #91793) donor vector and Kdm3b microhomology arms were cloned into the pCRIS-PITChv2-BSD-dTAG (Addgene #91792) donor vector to allow for dual selection with puromycin and blasticidin S hydrochloride. ..

    Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles
    Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS- FB2). .. Generating SON, SRRM2, and CTCF knock-in cell lines To generate an N- terminal Puro- P2A- dtag SON knock- in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro- P2A- dTAG, subcloned from pCRIS- PITChv2- Puro- dTAG, a gift from J Bradner and B Nabet (Addgene # 91793). ..

    Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles
    Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS-FB2). .. To generate an N-terminal Puro-P2A-dtag SON knock-in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro-P2A-dTAG, subcloned from pCRIS-PITChv2-Puro-dTAG, a gift from J Bradner and B Nabet (Addgene # 91793). ..

    Article Title: KDM3A and KDM3B Maintain Naïve Pluripotency Through the Regulation of Alternative Splicing
    Article Snippet: .. Briefly, Kdm3a microhomology arms were added to the pCRIS-PITChv2-Puro-dTAG (Addgene #91793) donor vector and Kdm3b microhomology arms were cloned into the pCRIS-PITChv2-BSD-dTAG (Addgene #91792) donor vector to allow for dual selection with puromycin and blasticidin S hydrochloride. ..

    Selection:

    Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells
    Article Snippet: .. Degron cell lines were generated following.31,32 Briefly, Kdm3a microhomology arms were added to the pCRIS-PITChv2-Puro-dTAG (Addgene #91793) donor vector and Kdm3b microhomology arms were cloned into the pCRIS-PITChv2-BSD-dTAG (Addgene #91792) donor vector to allow for dual selection with puromycin and blasticidin S hydrochloride. ..

    Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells
    Article Snippet: .. Degron cell lines were generated following., Briefly, Kdm3a microhomology arms were added to the pCRIS-PITChv2-Puro-dTAG (Addgene #91793) donor vector and Kdm3b microhomology arms were cloned into the pCRIS-PITChv2-BSD-dTAG (Addgene #91792) donor vector to allow for dual selection with puromycin and blasticidin S hydrochloride. ..

    Article Title: KDM3A and KDM3B Maintain Naïve Pluripotency Through the Regulation of Alternative Splicing
    Article Snippet: .. Briefly, Kdm3a microhomology arms were added to the pCRIS-PITChv2-Puro-dTAG (Addgene #91793) donor vector and Kdm3b microhomology arms were cloned into the pCRIS-PITChv2-BSD-dTAG (Addgene #91792) donor vector to allow for dual selection with puromycin and blasticidin S hydrochloride. ..

    Knock-In:

    Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles
    Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS- FB2). .. Generating SON, SRRM2, and CTCF knock-in cell lines To generate an N- terminal Puro- P2A- dtag SON knock- in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro- P2A- dTAG, subcloned from pCRIS- PITChv2- Puro- dTAG, a gift from J Bradner and B Nabet (Addgene # 91793). ..

    Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles
    Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS-FB2). .. To generate an N-terminal Puro-P2A-dtag SON knock-in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro-P2A-dTAG, subcloned from pCRIS-PITChv2-Puro-dTAG, a gift from J Bradner and B Nabet (Addgene # 91793). ..

    Amplification:

    Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles
    Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS- FB2). .. Generating SON, SRRM2, and CTCF knock-in cell lines To generate an N- terminal Puro- P2A- dtag SON knock- in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro- P2A- dTAG, subcloned from pCRIS- PITChv2- Puro- dTAG, a gift from J Bradner and B Nabet (Addgene # 91793). ..

    Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles
    Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS-FB2). .. To generate an N-terminal Puro-P2A-dtag SON knock-in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro-P2A-dTAG, subcloned from pCRIS-PITChv2-Puro-dTAG, a gift from J Bradner and B Nabet (Addgene # 91793). ..

    Construct:

    Article Title: Comparative cofactor screens show the influence of transactivation domains and core promoters on the mechanisms of transcription.
    Article Snippet: Eukaryotic transcription factors (TFs) activate gene expression by recruiting cofactors to promoters.. However, the relationships between TFs, promoters and their associated cofactors remain poorly understood.. Here we combine GAL4-transactivation assays with comparative CRISPR–Cas9 screens to identify the cofactors used by nine different TFs and core promoters in human cells.

    Polymerase Chain Reaction:

    Article Title: Comparative cofactor screens show the influence of transactivation domains and core promoters on the mechanisms of transcription.
    Article Snippet: Eukaryotic transcription factors (TFs) activate gene expression by recruiting cofactors to promoters.. However, the relationships between TFs, promoters and their associated cofactors remain poorly understood.. Here we combine GAL4-transactivation assays with comparative CRISPR–Cas9 screens to identify the cofactors used by nine different TFs and core promoters in human cells.



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