plasmid pcris pitchv2 puro dtag (Addgene inc)
93
Structured Review
Addgene inc
plasmid pcris pitchv2 puro dtag
Plasmid Pcris Pitchv2 Puro Dtag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcris+pitchv2+puro+dtag/pCRIS-PITChv2-Puro-dTAG+(BRD4)+(Plasmid+%2391793)/pm41904131-261-58-60
Average 93 stars, based on 36 article reviews
Plasmid Pcris Pitchv2 Puro Dtag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcris+pitchv2+puro+dtag/pCRIS-PITChv2-Puro-dTAG+(BRD4)+(Plasmid+%2391793)/pm41904131-261-58-60
Average 93 stars, based on 36 article reviews
plasmid pcris pitchv2 puro dtag - by Bioz Stars,
2026-10
93/100 stars
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Plasmid Preparation:Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells Article Snippet: .. Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS- FB2). .. Generating SON, SRRM2, and CTCF knock-in cell lines To generate an N- terminal Puro- P2A- dtag SON knock- in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro- P2A- dTAG, subcloned from Article Title: Targeting the mSWI/SNF complex in POU2F-POU2AF transcription factor-driven malignancies Article Snippet: LentiV_Cas9_puro , Tarumoto et al. , Addgene Plasmid #108100. .. Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS-FB2). .. To generate an N-terminal Puro-P2A-dtag SON knock-in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro-P2A-dTAG, subcloned from Generated:Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells Article Snippet: .. Degron cell lines were generated following.31,32 Briefly, Kdm3a microhomology arms were added to the Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells Article Snippet: .. Degron cell lines were generated following., Briefly, Kdm3a microhomology arms were added to the Clone Assay:Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells Article Snippet: .. Degron cell lines were generated following.31,32 Briefly, Kdm3a microhomology arms were added to the Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells Article Snippet: .. Degron cell lines were generated following., Briefly, Kdm3a microhomology arms were added to the Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS- FB2). .. Generating SON, SRRM2, and CTCF knock-in cell lines To generate an N- terminal Puro- P2A- dtag SON knock- in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro- P2A- dTAG, subcloned from Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS-FB2). .. To generate an N-terminal Puro-P2A-dtag SON knock-in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro-P2A-dTAG, subcloned from Article Title: KDM3A and KDM3B Maintain Naïve Pluripotency Through the Regulation of Alternative Splicing Article Snippet: .. Briefly, Kdm3a microhomology arms were added to the Selection:Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells Article Snippet: .. Degron cell lines were generated following.31,32 Briefly, Kdm3a microhomology arms were added to the Article Title: KDM3A and KDM3B regulate alternative splicing in mouse pluripotent stem cells Article Snippet: .. Degron cell lines were generated following., Briefly, Kdm3a microhomology arms were added to the Article Title: KDM3A and KDM3B Maintain Naïve Pluripotency Through the Regulation of Alternative Splicing Article Snippet: .. Briefly, Kdm3a microhomology arms were added to the Knock-In:Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS- FB2). .. Generating SON, SRRM2, and CTCF knock-in cell lines To generate an N- terminal Puro- P2A- dtag SON knock- in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro- P2A- dTAG, subcloned from Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS-FB2). .. To generate an N-terminal Puro-P2A-dtag SON knock-in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro-P2A-dTAG, subcloned from Amplification:Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS- FB2). .. Generating SON, SRRM2, and CTCF knock-in cell lines To generate an N- terminal Puro- P2A- dtag SON knock- in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro- P2A- dTAG, subcloned from Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles Article Snippet: HCT116 was cultured in McCoy’s 5A media (Thermo Fisher Scientific, 16600082) with 10% FBS (Peak Serum, PS-FB2). .. To generate an N-terminal Puro-P2A-dtag SON knock-in donor plasmid, SON homology arms of 1 kb were amplified from K562 genomic DNA and cloned into the ph1 backbone Puro-P2A-dTAG, subcloned from Construct:Article Title: Comparative cofactor screens show the influence of transactivation domains and core promoters on the mechanisms of transcription. Article Snippet: Eukaryotic transcription factors (TFs) activate gene expression by recruiting cofactors to promoters.. However, the relationships between TFs, promoters and their associated cofactors remain poorly understood.. Here we combine GAL4-transactivation assays with comparative CRISPR–Cas9 screens to identify the cofactors used by nine different TFs and core promoters in human cells. Polymerase Chain Reaction:Article Title: Comparative cofactor screens show the influence of transactivation domains and core promoters on the mechanisms of transcription. Article Snippet: Eukaryotic transcription factors (TFs) activate gene expression by recruiting cofactors to promoters.. However, the relationships between TFs, promoters and their associated cofactors remain poorly understood.. Here we combine GAL4-transactivation assays with comparative CRISPR–Cas9 screens to identify the cofactors used by nine different TFs and core promoters in human cells. |